HF 8311

Sperm Cryopreservation at Generations Fertility Care

Sperm cryopreservation is a process to collect, freeze and store your sperm for possible use in the future. Sperm are reproductive cells produced by the testicles and ejaculated from the penis during sexual activity. Cryopreservation allows patients to preserve or “bank” sperm for health conditions, frequent traveling, military deployment, prior to cancer treatment, vasectomy, or gender affirming treatment.

Cryopreserved sperm can be used to achieve a pregnancy through fertility treatments including intrauterine insemination (IUI) or in vitro fertilization (IVF). For IUI, sperm are thawed and inserted into the uterus to attempt pregnancy. For IVF, sperm are thawed and combined with eggs outside the body, and the embryo is returned to the uterus to attempt pregnancy.

If you do not follow these instructions, your sample may be rejected.

Before Sperm Cryopreservation

You must be an established patient at Generations to have your sperm cryopreserved in the Andrology Laboratory. If you are not an established patient, call Generations at (608) 824-6160 to schedule your initial visit. The clinic is located at 2365 Deming Way, Middleton, WI 53562.


  • You must have a consult appointment with a Generations provider before a consent and collection appointment.

  • The Andrology Laboratory must receive a physician requisition before your collection.

  • You will be scheduled for a consent appointment and a semen collection appointment on the same day.

  • Available appointment times are Monday through Friday 7:30 am to 11:00 am.

  • At the consent appointment, your provider will discuss the risks and benefits of sperm cryopreservation, and fees.

  • After your consent appointment, you will collect semen in a private collection room.

  • Bring a valid government issued photo ID card to your appointment.

  • Infectious Disease Testing

  • Before you store your sperm, you must be tested for the following infectious diseases:

    • Hepatitis B Surface Antigen

    • Hepatitis B Core Antibody

    • Hepatitis C Antibody

    • HIV I/II Antibody

    • HTLV I/II

    • Syphilis

    • Gonorrhea/Chlamydia

  • If your testing is done outside of Generations, please have your physician’s office fax the results to Generations at 608-827-3040.

Days of Abstinence

  • Semen should be collected by masturbation after 2-3 days of abstinence (no sexual intercourse, masturbation, or ejaculation).

  • Longer periods of abstinence, greater than 7 days, should be avoided, as this may increase the number of dead sperm in the semen.

Collection Containers

  • Semen sample must be collected into the sterile container provided by Generations.

  • Do not collect the semen in any other container, as it may result in decreased survival of the sperm.

Collection Method

  • Semen samples should be collected by masturbation.

  • Do not use condoms or lubricant gel. These will kill the sperm in the semen.

  • The entire semen sample must be collected in the container, especially the first part of the sample.

  • A special nontoxic condom collection kit is available at Generations if you do not want to masturbate.

Collection Locations

  • Semen samples can be collected at Generations or at your home.

  • Private rooms dedicated to semen collection are available at Generations.

  • Semen samples collected at home should be dropped off at Generations within one hour of collection but must be dropped off within two hours of collection.


  • The sample is evaluated for consistency, sperm count, sperm motility and sperm shape.

  • After the evaluation, the sample is diluted with a cryoprotectant to protect the sperm during cryopreservation.

  • The sample is then put in vials and frozen.

  • The samples are stored at -196°C and may be stored indefinitely at this temperature.


  • Results will be available in MyChart within 7 to 10 days after your appointment.

  • Results are automatically sent to your provider for review.


  • All patients must register with ReproTech before their semen cryopreservation appointment.

  • Generations is a member of the ReproTech, LTD Fertility Preservation Network. The Network is based on ReproTech’s long history in sperm banking and their leadership and expertise in long term storage.

  • Registration information can be found at: https://www.reprotech.com/forms

  • All cryopreserved sperm will be transferred to long term storage at ReproTech.